兰州大学

学习经历

1995 美国弗吉尼亚理工大学 博士 (Ph.D. Virginia Tech, 1995)
1989 中国科学院植物研究所 硕士 (M.S. Institute of Botany, CAS, 1989)
1984 兰州大学生物系 学士 (B.S. Lanzhou University, 1984 )

工作经历

1984-1986年在湖南师范大学生物系任助教。1990-1991年在中科院植物研究所任助研。1997-2002年在美国密苏里大学哥伦比亚分校从事博士后研究。2002年8月起在美国俄克拉荷马大学植物与微生物系任助理教授，2008年5月晋升为副教授，获终身教职（tenured）。通过全球竞聘获任兰州大学生命科学学院院长，于2009年3月全职回国工作。是国家"****"特聘专家，教育部“长江学者”特聘教授。获2013-2014年度美国弗吉尼亚理工大学农学与生命科学学院杰出校友奖。

教学及指导研究生情况

给研究生讲授“Plant Hormones”; 目前指导3名博士后，16名博士研究生、16名硕士研究生。

发表论文及专著

Selected Publications:

Guo, X., Qin, Q., Yan, J., Niu, Y., Huang, B., Guan, L., Li, Y., Ren, D.,Li, J., Hou, S. (2015) TYPE-ONE PROTEIN PHOSPHATASE 4 regulates pavement cell interdigitation by modulating PIN-FORMED 1 polarity and trafficking in Arabidopsis. Plant Physiology 167 (3), 1058-1075.

Qin, Q., Wang, W., Guo, X., Yue, J., Huang, Y., Xu, X., Li, J., Hou, S. (2014) Arabidopsis DELLA Protein Degradation Is Controlled by a Type-One Protein Phosphatase, TOPP4. PLoS Genet 10(7):e1004464. doi: 10.1371/journal.pgen.1004464.

Yang, Z.R., Zhang, C.J., Yang, X.J., Kun, L.,Wu, Z.X., Zhang, X.Y., Zheng, W.,Xun, Q.Q., Liu, C.L., Lu, L.L., Yang, Z.E., Qian, Y.Y., Xu, Z.Z.,Li, C.F., Li,J.*, Li, F.G.* (2014) PAG1, a cotton brassinosteroid catabolism gene, modulates fiber elongation. New Phytologist 203, 437-448. (* co-corresponding authors)

Qin, Z.X., Zhang, X.R., Zhang, X., Xin, W., Li, J., Hu, Y.X. (2014) The Arabidopsis transcription factor IIB-related protein BRP4is involved in the regulation of mitotic cell-cycle progression during male gametogenesis. Journal of Experimental Botany 65(9),2521-2531.

He, K., Xu, S., Li, J. (2013) BAK1 Directly Regulates Brassinosteroid Perception and BRI1 Activation. Journal of Integrative Plant Biology 55(12),1264-1270.

Li, J., Tax, F.E. (2013) Receptor-Like Kinases: Key Regulators of Plant Development and Defense. Journal of Integrative Plant Biology 55(12),1184-1187.

Ma, T. et al. (2013) Genomic insights into salt adaptation in a desert poplar. Nature Communications 4, 2797.

Qian, P., Han, B., Forestier, E., Hu, Z., Gao, N., Lu, W., Schaller, H., Li, J., Hou, S. (2013) Sterols are required for cell fate commitment and maintenance of the stomatal lineage in Arabidopsis. The Plant Journal 74, 1029-1044.

Zhao, B. and Li, J. (2012) Regulation of Brassinosteroid Biosynthesis and Inactivation. Journal of Integrative Plant Biology 54 (10), 746-759.

Wen, J., Li, J., and Walker, J.C. (2012) Overexpression of a Serine Carboxypeptidase Increases Carpel Number and Seed Production in Arabidopsis thaliana. Food and Energy Security 1(1), 61-69.

Du, J., Yin, H., Zhang, S., Wei, Z., Zhao, B., Zhang, J., Gou, X., Lin, H.*, and Li, J. (2012) Somatice Embryogenesis Receptor Kinases Control Root Development Mainly via Brassinosteroid-Independent Actions in Arabidopsis thaliana. Journal of Integrative Plant Biology54(6), 388-399.

Gou, X. and Li, J. (2012) Activation tagging. Methods in Molecular Biology 876, 117-133.

Gou, X., Yin, H., He, K., Du, J., Yi, J., Xu, S., Lin, H., Clouse, S.D., Li, J. (2012) Genetic Evidence for an Indispensable Role of Somatic Embryogenesis Receptor Kinases in Brassinosteroid Signaling. PLoS Genet 8(1): e1002452. doi:10.1371/journal.pgen.1002452

Li, J., Du, J., He, K., and Gou, X. (2012) Cell Death Control by Receptor Kinases in Arabidopsis thaliana. In, F.E. Tax and B. Kemmerling (editors) RECEPTOR-LIKE KINASES IN PLANTS: From Development to Defense. Springer. Volume 13, pp79-91.

Wei, Z-Y and Li, J. (2011) Receptor kinases mediated brassinosteroid signal transduction in plants. Chinese Bulletin of Life Sciences. 23 (11), 1106-1113.

An, J., Guo, Z., Gou, X., Li, J. (2011) TCP1 positively regulates the expression of DWF4 in Arabidopsis thaliana. Plant Signaling & Behavior, 6: 1117-1118.

Li, J.（2010） Multi-tasking of somatic embryogenesis receptor-like protein kinases. Curr Opin Plant Biol ,13: 509-514

Guo, Z., Fujioka, S., Blancaflor, E.B., Miao, S., Gou, X., and Li, J. (2010) TCP1 modulates brassinosteroid biosynthesis by regulating the expression of the key biosynthetic gene DWARF4 in Arabidopsis thaliana. Plant Cell 22, 1161-1173.

Yang, H., Gou, X., He, K., Xi, D., Du, J., Lin, H., Li, J. (2010) Arabidopsis BAK1 and BKK1 confer reduced susceptibility to Turnip Crinkle Virus. European Journal of Plant Pathology, 127: 147-156.

Gou, X., He, K., Yang, H., Yuan, T., Lin, H., Clouse, S.D., Li, J. (2010) Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana. BMC Genomics 11(1):19

Li, D., Wang, M., Xu Y.Y., Luo, W., Liu, Z.H., Li, J., Chong, K.(2009) Engineering OsBAK1 gene as a molecular tool to improve rice architecture for high yield. Plant Biotechnol Journal 7(8): 791-806.

Wang, X., Kota, U., He, K., Blackburn, K., Li, J., Goshe, M.B., Huber, S.C., and Clouse, S.D.(2008) Sequential transphosphorylation of the BRI1/BAK1 receptor kinase pair impacts early events in brassinosteroid signaling. Developmental Cell 15, 220-235.

He, K., Gou, X., Powell, R.A., Yang, H., Yuan, T, Guo, Z., and Li, J. (2008) Receptor-like protein kinases, BAK1 and BKK1, regulate a light-dependent cell-death control pathway. Plant Signaling & Behavior 3 (10), 813-815.

Clouse, S.D., Goshe, M.B., Huber, S.C. and Li, J. (2008) Functional Analysis and Phosphorylation Site Mapping of Leucine-Rich Repeat Receptor-Like Kinases. In, GK Agrawal and R Rakwal (eds) Plant Proteomics: Technologies, Strategies and Applications. John Wiley & Sons, pp 469-484.

Heese, A., Hann, D.R., Gimenez-ibanex, S., Jones, A., He, K., Li, J., Schroeder, J.I., Peck, S.C., Rathjen, J.P. (2007) The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants. Proc. Natl. Acad. Sci. USA 104, 12217-12222.

He, K., Gou, X., Yuan, T., Lin, H., Asami, T., Yoshida, S., Russell, S.D., Li, J. (2007) BAK1 and BKK1 regulate brassinosteroid-dependent growth and brassinosteroid-independent cell death pathways. Current Biology 17, 1109-1115.

Yuan, T., Fujioka, S., Takatsuto, S., Matsumoto, S., Gou, X.,He, K., Russell, S.D., and Li, J. (2007) BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana. The Plant Journal 51 (2), 220–233.

Li, J. and Gou, X. (2007) Brassinosteroids. In: Encyclopedia of Life Sciences. John Wiley & Sons, Ltd: Chichester http://www.els.net/ [DOI: 10.1002/9780470015902.a0020092]

Wang, L., Xu, Y.Y., Li, J., Powell, R.A., Xu, Z.H., and Chong, K. (2007) Transgenic rice plants ectopically expressing AtBAK1 are semi-dwarfed and hypersensitive to 24-epibrassinolide. Journal of Plant Physiology 164(5), 655-664.

Zhou, A. and Li, J. (2005) Arabidopsis BRS1 is an active and secreted serine carboxypeptidase. Journal of Biological Chemistry 280 (42), 35554-35561.

Xu, Y.Y., Wang, X.M., Li, J., Li, J.H., Wu, J.S., Walker, J.C., Xu, Z.H., Chong, K. (2005) Activation of the WUS gene induces ectopic initiation of floral meristems on mature stem surface in Arabidopsis thaliana.Plant Molecular Biology 57 (6), 773-784.

Wang, X.F., Goshe, M. B., Soderblom, E., Phinney, B.S., Kuchar, J., Li, J., Asami, T., Yoshida, S., Huber, S. C., Clouse, S. D. (2005) Identification and functional analysis of in vivo phosphorylation sites of the Arabidopsis BRASSINOSTEROID-INSENSITIVE 1 receptor kinase. The Plant Cell 17(6), 1685-703.

Zhou, A., Wang, H., Walker, J.C., and Li, J. (2004) BRL1, a leucine-rich repeat receptor-like protein kinase, is functionally redundant with BRI1 in regulating Arabidopsis brassinosteroid signaling. The Plant Journal 40, 399-409.

Lee, G., Li, J., Walker, J.C. and Van Doren S.R. (2003) 1H. 13C and 15N resonance assignments of the kinase interacting FHA domain of Arabidopsis thaliana kinase-associated protein phosphatase.J. Biomol NMR 25, 253-254.

Li, J., Wen, J., Lease, K.A., Doke, J.T., Tax, F.E. and Walker, J.C. (2002) BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. Cell 110, 213-222.

Lease, K.A., Wen, J., Li, J., Doke, J.T., Liscum, E. and Walker, J.C. (2001) A mutant Arabidopsis Heterotrimeric G protein b subunit affects leaf, flower and fruit development. The Plant Cell 13, 2631-2641.

Li, J., Lease, K.A., Tax, F.E. and Walker, J.C. (2001) BRS1, a serine carboxypeptidase, regulates BRI1 signaling in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 98, 5916-5921.

Li, J., Lee, G., Van Doren, S.R. and Walker, J.C. (2000) The FHA domain mediates phosphorylation-dependent protein-protein interactions. J. Cell Sci. 113, 4143-4149.

Li, J., Smith, G.P. and Walker, J.C. (1999) Kinase interaction domain of kinase-associated protein phosphatase, a phosphoprotein-binding domain. Proc. Natl. Acad. Sci. USA 96, 7821-7826.

Lin, Q., Li, J., Smith, R.D. and Walker, J.C. (1998) Molecular cloning and chromosomal mapping of type one serine/threonine protein phosphatases in Arabidopsis thaliana. Plant Mol. Biol. 37, 471-481.

Li, J., Hegeman, C.E., Hanlon, W.R., Lacy, G.H., Denbow, D.M. and Grabau, E.A. (1997) Secretion of active recombinant phytase from soybean cell-suspension cultures.Plant Physiol. 114, 1103-1111.

Li, J. and Grabau, E.A. (1996) Comparison of somatic embryogenesis and embryo conversion in commercial soybean cultivars. Plant Cell, Tissue Organ Cult. 44, 87-89.

研究方向

1 ）油菜素内酯的信号传递、合成、及代谢途径的分子调控机制 (Brassinosteroid Signal Transduction and Homeostasis): 油菜素内酯是一类重要的植物激素，参与调控植物生长的各个阶段，阻断油菜素内酯的生物合成或信号转导都会使植物变得极其矮小，并表现出雄性不育、推迟衰老、在黑暗培养时呈现出去黄化等的异常表型。油菜素内酯的受体是一个跨膜的亮氨酸富集型受体激酶，它的胞外特定结构域能特意与油菜素内酯相互作用从而启动胞内一系列磷酸化、脱磷酸化反应，把信号一步步传到细胞核中，最终改变基因的表达。在过去的十年中，本研究小组利用激活标签遗传学手段成功地分离到多个关键蛋白 ( 比如： BRS1 ， BAK1 ， AtSERK1 ， BRL1 ， BRL3 ， BEN1 等 ) ，研究表明它们参与油菜素内酯的信号转导、合成、及代谢调控。利用经典遗传学及生物化学手段，本实验室清楚地证明了BAK1在油菜素内酯信号早期阶段起必不可少的作用。现阶段的工作集中在利用遗传、生化、蛋白组学等方法继续发现参与油菜素内酯信号转导、合成、及代谢调控的新蛋白，并对以往实验室已发现蛋白所参与的具体作用机制作深入细致的探讨。同时，我们也密切关注不同植物激素之间通过互作来调控植物的生长发育及逆境适应的分子机理。

2 ）用遗传及生化手段研究拟南芥中一类亮氨酸富集型受体蛋白激酶的生物学功能 (Functional Analyses of LRR-RLKs): 细胞与细胞之间的信号转导是高等植物生长发育的细胞生物学基础，受体激酶在信号转导过程中起至关重要的作用，它们通常作为信号转导的原初受体。过去的十几年中本实验室做了大量的前期工作，克隆了拟南芥基因组中全部223个编码亮氨酸富集型受体蛋白激酶的大多数全长 cDNA ，克隆并建立了全部223个LRR-RLK的promoter-GUS转基因植株，对它们在不同发育阶段的组织水平的转录表达进行了详细的研究，对进一步了解它们真正的生物学功能奠定了良好的基础。目前本实验室正在利用各种遗传学、分子生物学及组学手段对223LRR-RLKs中尚未揭示功能的分子开展生物学功能研究，已有多个尚未研究过的LRR-RLKs的功能被鉴定到，它们的具体分子机理正在研究之中。

3 ）受体激酶介导的植物细胞凋亡的分子机理 (RLK-Mediated Plant Cell-Death Control): 我们前面的工作已发现受体激酶 BAK1 及 BKK1 能同时参与正向调控油菜素内酯信号转导，同时抑制细胞凋亡，同时敲除 BAK1 和 BKK1 使植物变得极其矮小，并在光下很快进入细胞凋亡过程，造成叶片快速死亡。我们目前正在对 BAK1 及 BKK1 调控细胞凋亡的分子机理作深入的探索。通过传统遗传学手段，我们已发现了多个能抑制双突变体bak1 bkk1细胞死亡的遗传抑制子，它们的具体作用机制还在研究中。

项目成果

科研项目：

1）国家基金委重大研究计划重点项目“TCP类转录因子在调控油菜素内酯生物合成及信号转导中的作用机理”， 90917019，2010.1.1到2013.12.31， 课题负责人：黎家

2）国家基金委重大研究计划集成项目“油菜素内酯生物合成调控的分子机理”，91117008， 2012.1.1到2013.12.31，课题负责人： 黎家

3）科技部国家基础研究发展计划“高等植物蛋白质修饰与降解调控的分子机理研究”项目（项目负责人：谢旗）中的课题之一“植物蛋白的修饰的重要过程与调控机理”，2011CB915401，2011.1.1到2015.12.31, 课题负责人：黎家

4）国家基金委重大研究计划集成项目“BAK1调控油菜素内酯信号转导及器官发育的分子机制”，91317311，2014.1.1到2015.12.31，课题负责人: 黎家

荣誉、获奖 Virginia Tech农学与生命科学学院杰出校友奖（2013-2014年度）； 兰州大学“师德标兵”（2013）；中组部“****”特聘专家（2012年）；科学中国人（2011年）年度人物奖； 甘肃省领军人才（2010年，2014年）； 教育部 "长江学者奖励计划"特聘教授 （2008年）；兰州大学“萃英”特聘教授（2009）；Oklahoma 大学青年教师奖（2003）。

社会工作

中国植物生理学会常务理事, 中国细胞生物学会理事, Food and Energy Security 副主编, Journal of Integrative Plant Biology 编委,“细胞活动与逆境适应”教育部重点实验室主任等

其它信息

CONTACT INFORMATION:
Dr. Jia Li, School of Life Sciences, Lanzhou University, 222 South Tianshui Road, Lanzhou 730000, People's Republic of China

Lab web page: www.lilab.cn; Email: lijia@lzu.edu.cn; Office phone: 0931-8915662